[ensembl-dev] Failed to get Ensembl genes with the API
Sebastien Moretti
smoretti at unil.ch
Tue Jan 5 12:57:32 GMT 2021
Hi Mag
ports 5306 and 3306 should be similar according to
http://www.ensembl.org/info/data/mysql.html
Any differences if 'mus_musculus' is used instead of 'mus musculus'?
Best
Sébastien
> Hi Mag
>
>> Hi Sébastien,
>>
>> Thank you for bringing this to our attention.
>> If you are only wanting to access data for mouse, the 'api' alias for
>> git ensembl will provide all the necessary repositories.
>> You are right however that ensembl-metadata and ensembl-taxonomy
>> should be made more easily accessible through our Git tools and we
>> will update this in the coming release.
>
> Thanks
>
>> Regarding your issues retrieving mouse genes, could you provide more
>> details on your script?
>>
>> The following printed out 56,305.
>>
>> my $registry = "Bio::EnsEMBL::Registry";
>>
>> $registry->load_registry_from_db(
>> -db_version => 102,
>> -host => 'ensembldb.ensembl.org',
>> -user => 'anonymous',
>> -port => 3306
>> );
>>
>> my $gene_adaptor = $registry->get_adaptor( 'mus musculus', 'Core',
>> 'Gene' );
>>
>> my $genes = $gene_adaptor->fetch_all();
>> print "Found " . scalar(@$genes) . "\n";
>
> Our script is similar with API version 102:
> my $reg = 'Bio::EnsEMBL::Registry';
> $reg->load_registry_from_db( -user => 'anonymous',
> -pass => '',
> -host => 'ensembldb.ensembl.org',
> -port => 5306,
> -verbose => 1, # Verbose output
> );
> my $gene_adaptor = $reg->get_adaptor( 'mus_musculus', 'Core', 'Gene' );
> my @genes = @{ $gene_adaptor->fetch_all() };
>
>
> The main differences are "mus_musculus" instead of "mus musculus"
> and port 5306 instead of 3306.
>
>
> Cheers
> Sébastien
>
>> Cheers,
>> mag
>>
>> On 15/12/2020 16:44, Sebastien Moretti wrote:
>>> Hi
>>>
>>> Previously I got all Ensembl gene for a species with the API this way:
>>> $gene_adaptor = $reg->get_adaptor( 'mus musculus', 'Core', 'Gene' );
>>>
>>>
>>> But it does not look to be so simple with more recent Ensembl API
>>> versions.
>>> First I use the "Git Ensembl tools" but ensembl-metadata and
>>> ensembl-taxonomy modules did not come with it for [api].
>>> According to what is written at
>>> http://www.ensembl.org/info/docs/api/api_installation.html I expected
>>> them to come with.
>>>
>>> http://www.ensembl.org/info/docs/api/api_git.html mentions
>>> ensembl-metadata.git but it is not there unless you do
>>> git ensembl --clone available
>>> instead of
>>> git ensembl --clone api
>>>
>>>
>>>
>>> Second what is the right way nowadays to get adaptor for all genes?
>>> $gene_adaptor = $reg->get_adaptor( 'mus musculus', 'Core', 'Gene' );
>>> looks to work but returns only a subset of genes (between 20 and 70).
>>>
>>> Thanks for your help
>>>
>>> --
>>> Sébastien Moretti
>>> Staff Scientist
>>> Department of Ecology and Evolution,
>>> Biophore, University of Lausanne,
>>> CH-1015 Lausanne, Switzerland
>>> Tel.: +41 (21) 692 4221/4079
>>> http://bioinfo.unil.ch/ http://bgee.org/ http://selectome.unil.ch/
>>>
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