[ensembl-dev] Failed to instantiate plugin Conservation: Failed to connect to compara database
Guillermo Marco Puche
guillermo.marco at sistemasgenomicos.com
Thu Mar 9 16:33:19 GMT 2017
Thank you Will! Indeed I had a problem with ensembl-compara API local
install.
Regards,
Guillermo
On 03/08/2017 04:48 PM, Will McLaren wrote:
> OK, next thing to check is that your ensembl-compara API checkout is
> up to date (it should be on release/87).
>
> Will
>
> On 8 March 2017 at 15:25, Guillermo Marco Puche
> <guillermo.marco at sistemasgenomicos.com
> <mailto:guillermo.marco at sistemasgenomicos.com>> wrote:
>
> Hi Will,
>
> My bad that '-host' was a typo when posting command to dev mail
> list. I've actually tested it with both local database and remote
> database and I'm getting the same error. However this is the
> corrected command I'm testing right now:
>
> variant_effect_predictor.pl <http://variant_effect_predictor.pl> -i test.vcf -o test_out.vcf -config /home/ensembl_test/87.conf -dir_plugins /home/ensembl_test -dir_cache /share/references/vep -host*ensembldb.ensembl.org <http://ensembldb.ensembl.org>* -fork 4
>
> Regards,
> Guillermo.
>
>
> On 03/08/2017 04:13 PM, Will McLaren wrote:
>> Hi Guillermo,
>>
>> You have nothing following your "-host" entry in your command
>> line; odd that this doesn't cause any other errors, but probably
>> the root of your issue.
>>
>> Regards
>>
>> Will McLaren
>> Ensembl Variation
>>
>> On 7 March 2017 at 08:25, Guillermo Marco Puche
>> <guillermo.marco at sistemasgenomicos.com
>> <mailto:guillermo.marco at sistemasgenomicos.com>> wrote:
>>
>> Dear devs,
>>
>> I'm having trouble running Conservation plugin on latest
>> Ensembl 87 version. Here's the log:
>>
>> variant_effect_predictor.pl <http://variant_effect_predictor.pl> -i test.vcf -o test_out.vcf -config /home/ensembl_test/87.conf -dir_plugins /home/ensembl_test -dir_cache /share/references/vep -host -fork 4
>>
>> 2017-03-07 09:15:31 - Read configuration from /home/ensembl_test/87.conf
>> 2017-03-07 09:15:44 - Read existing cache info
>> 2017-03-07 09:15:44 - Auto-detected FASTA file in cache directory***2017-03-07 09:18:27 - Failed to instantiate plugin
>> Conservation: Failed to connect to compara database*
>> 2017-03-07 09:18:34 - Starting...
>> 2017-03-07 09:18:34 - Detected format of input file as vcf
>> 2017-03-07 09:18:34 - Read 51 variants into buffer
>> 2017-03-07 09:18:34 - Calculating consequences
>> 2017-03-07 09:18:36 - Writing output
>> 2017-03-07 09:18:36 - Processed 51 total variants (26 vars/sec, 26 vars/sec total)
>> 2017-03-07 09:18:36 - Finished!
>>
>>
>> I've cloned it from VEP plugin repo just to be sure I've
>> latest version. I've tested it against both local Ensembl DB
>> installation and remote host both failing.
>>
>> This is my config file:
>>
>> cache 1
>> plugin Conservation
>> force_overwrite 1
>> no_escape 1
>> no_progress 0
>> no_stats 1
>> hgvs 1
>> canonical 1
>> symbol 1
>> ccds 1
>> check_existing 1
>> allele_number 1
>> check_alleles 1
>> domains 1
>> gmaf 1
>> maf_1kg 1
>> maf_esp 1
>> numbers 1
>> uniprot 1
>> biotype 1
>> tsl 1
>> pubmed 1
>> polyphen b
>> regulatory 1
>> sift b
>> xref_refseq 1
>> vcf 1
>>
>> Regards,
>> Guillermo.
>>
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>>
>>
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