[ensembl-dev] GRCh37 Protein sequence has asterisks
Alessandro Vullo
avullo at ebi.ac.uk
Mon Dec 4 17:06:49 GMT 2017
Hi Luke,
thanks for reporting this, we're currently investigating the issue.
best,
Alessandro
On 04/12/17 15:57, Luke Goodsell wrote:
> Hi,
>
> I’ve been trying to retrieve some GRCh37 protein sequences with code
> similar to that below and have found some proteins that have corrupted
> sequences, including asterisks. Please can you tell me how to get the
> correct protein sequences?
>
> #!/usr/bin/env perl
>
> usestrict;
>
> usewarnings;
>
> useBio::EnsEMBL::Registry;
>
> useGetopt::Long qw(GetOptionsFromArray);
>
> my$registry = 'Bio::EnsEMBL::Registry';
>
> $registry->load_registry_from_db(
>
> -host => 'ensembldb.ensembl.org',
>
> -user => 'anonymous',
>
> -port => '3337'
>
> );
>
> my$transcript_adaptor = $registry->get_adaptor( 'Human',
> 'otherfeatures', 'Transcript');
>
> foreachmy$transcript_id ("NM_002426.4") {
>
> foreachmy$transcript (@{
> $transcript_adaptor->fetch_all_versions_by_stable_id($transcript_id) }) {
>
> my$protein = $transcript->translation();
>
> defined($protein) ornext;
>
> print">". $protein->stable_id() . "\n";
>
> print$protein->seq() . "\n";
>
> }
>
> }
>
> Kind regards,
>
> Luke
>
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--
Alessandro Vullo, PhD
Software Engineer, Ensembl
European Bioinformatics Institute (EMBL-EBI)
http://www.ebi.ac.uk
http://www.ensembl.org
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