[ensembl-dev] chain files to build cross-species coordinates conversion (liftover)
Andrew Yates
ayates at ebi.ac.uk
Tue Apr 14 15:14:30 BST 2015
Hi Juan
That seems to be a mistake on our website documentation. All chain files
used for our assembly mapper are available from
ftp://ftp.ensembl.org/pub/assembly_mapping/
As others have mentioned this provides chain files for mapping between
species assemblies and not between species. You would have to follow the
information Matthieu has provided separately to get this information
Andy
Juan Pascual Anaya wrote:
> Hi Anne,
>
> Thank you very much for your reply!
>
> According to those links that I sent about the pairwise alignments I'm
> interested in, you can read the following:
>
> "After running LastZ, the raw LastZ alignment blocks are chained
> according to their location in both genomes. During the final netting
> process, the best sub-chain is chosen in each region on the reference
> species."
>
> So, I thought that those chain files were already done and available
> somewhere.
>
> I'm using all my genome coordinates from Ensembl (e74), so I guess I
> could also use your Assembly Converter tool, but I would still need the
> chain files, right? Here:
>
> http://www.ensembl.org/info/docs/webcode/mirror/tools/assembly_converter.html
>
> when I try to go to the FTP site where the "current chain files" are,
> the site doesn's exist, so I thought that this method is out of date.
>
> If the Compara team has more info about this, that would be great!
>
> Thanks!
> Juan
>
>
>
> On Mon, Apr 13, 2015 at 8:00 PM, <dev-request at ensembl.org
> <mailto:dev-request at ensembl.org>> wrote:
>
>
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>
> Message: 2
> Date: Mon, 13 Apr 2015 09:24:21 +0100
> From: Anne Lyle <annelyle at ebi.ac.uk <mailto:annelyle at ebi.ac.uk>>
> Subject: Re: [ensembl-dev] chain files to build cross-species
> coordinates conversion (liftover)
> To: Ensembl dev list <dev at ensembl.org <mailto:dev at ensembl.org>>
> Message-ID: <326CB180-BB1F-4218-90E6-722656215245 at ebi.ac.uk
> <mailto:326CB180-BB1F-4218-90E6-722656215245 at ebi.ac.uk>>
> Content-Type: text/plain; charset="utf-8"
>
> Hi Juan
>
> We currently only produce chain files (for our own Assembly
> Converter) for those species where we?ve done an assembly mapping
> through our core pipeline. Perhaps our compara team can chime in
> with whether it?s feasible to create them based on pairwise alignments.
>
> Cheers
>
> Anne
>
>
> > On 12 Apr 2015, at 09:42, Juan Pascual Anaya
> <jpascualanaya at gmail.com <mailto:jpascualanaya at gmail.com>> wrote:
> >
> > Hi Ensembl team,
> >
> > I am currently doing a comparative analysis of some ChIP-seq
> datasets between mouse, chicken and turtle (P. sinensis). Therefore,
> I was interested in getting a cross-species coordinate conversion,
> like assembly converter or liftover. I've seen that you have these
> two pairwise alignments done:
> >
> > Mouse-Chicken pairwise alignment
> > http://www.ensembl.org/info/genome/compara/mlss.html?mlss=633
> <http://www.ensembl.org/info/genome/compara/mlss.html?mlss=633>
> >
> > Chicken-Turtle pairwise alignment
> > http://www.ensembl.org/info/genome/compara/mlss.html?mlss=657
> <http://www.ensembl.org/info/genome/compara/mlss.html?mlss=657>
> >
> > So, I was wondering if I could get the best subset of chains from
> your LASTZ-net results (i.e., the liftover chain files) so that I
> can do myself a liftOver. I have not seen this files for download in
> the Ensembl webpage, so could you let me know how to get them?
> >
> > Thank you very much,
> > Juan
> >
> > _______________________________________________
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> --
>
> Juan Pascual-Anaya, PhD
> Research Scientist
> Evolutionary Morphology Laboratory, RIKEN
> 2-2-3 Minatojima-minamimachi
> Chuo-ku, Kobe, Hyogo 650-0047
> Japan
>
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--
Andrew Yates - Genomics Technology Infrastructure Team Leader
European Molecular Biology Laboratory
European Bioinformatics Institute
Wellcome Trust Genome Campus
Hinxton, Cambridge
CB10 1SD, United Kingdom
Tel: +44-(0)1223-492538
Fax: +44-(0)1223-494468
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