[ensembl-dev] Ensembl Genomes BLAST: Parsing failed ERROR

Rafael Hernández rafahd87 at gmail.com
Tue Mar 5 10:13:33 GMT 2013


Hi,

So if I want to use BLAST in my LOCAL Ensembl Genome, Should the machine be
connected to internet to access to EBI Blast service?
Then, I've got a big trouble because the machine won't be connected to
internet...
But Why for Ensembl is it possible to use local NCBI Blast and for EG not?

And, Which EBI BLAST tool is been used through those web services? I
suppose that EG use wu-blast not ncbiblast, and this is the reason that
make the parsing step crash (because a wu_blast output is expected).
I could try to change the scripts to use a local version of wu_blast
instead the web services however wu_blast sources (as far as I know) are
not available for downloading since all rights were acquired by Advanced
Biocomputing. There is any place where I can find a open version of
wu-blast?

Another option could be use NCBI Blast without the BLAST plugin (as Ensembl
does), but then EG Blastview fails and I've got again the problem of empty
comboboxes.....

Please help me, I need to solve it ASAP, it should be working before next
week.

Thank you and regards,


Rafa Hernández de Diego

SLU-Global Bioinformatics Center
Swedish University for Agricultural Sciences (SLU)
Gerda Nilssons väg 2,
 Box 7023, 750 07 Uppsala, Sweden.
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