[ensembl-dev] Is it a template or coding strand? when given Transcript coordinates.
Allan Kamau
kamauallan at gmail.com
Wed Nov 28 08:32:28 GMT 2012
When I obtain transcript location and strand from EnsEMBL for a given
transcript for example "ENST00000217246" should I assume the location is
always the template strand and that the transcription product would be
equivalent to the coding strand (the complement of the transcript's
sequence nucleotides)?
For a given transcript, I am trying to obtain to generate the immediate RNA
sequence produced by the transcription mechanism for the given transcript.
Should I simply generate these data by obtaining the transcript's sequence
then complementing each nucleotide, then substitute each T with a U?
Allan.
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