[ensembl-dev] Missing IDS in ENSEMBL database
Thibaut Hourlier
th3 at sanger.ac.uk
Fri May 25 11:19:54 BST 2012
Dear Duarte,
I went through the four first of your IDs:
On 25 May 2012, at 10:14, Duarte Molha wrote:
> Dear Developers
>
> I created a simple script to output the exons of specific transcripts with NM ids.
> It works fine for all but a small list of IDS. The large majority of the failed IDS have been suppressed from NCBI
> Because they were found to be a “ nonsense-mediated mRNA decay (NMD) candidate” so I do not mind eliminating those records from my query.
>
> However some of the ones that fail are in NCBI database and for some reason ENSEMBL is not able to query them:
> NM_001040409.1
It is an NMD transcript.
> NM_001167607.1
It is an exon supporting feature and not a transcript supporting feature, i think this is the reason you don't get it with your script
http://www.ensembl.org/Homo_sapiens/Transcript/SupportingEvidence?db=core;g=ENSG00000196743;r=5:150591711-150650001;t=ENST00000523466
> NM_001199987.1
If you look in the Gene database at NCBI you will see that there is 2 other sequences for NDUFB6, which are the transcript supporting feature for the 2 transcripts in Ensembl for the gene.
http://www.ncbi.nlm.nih.gov/gene/?term=NM_001199987.1
http://www.ensembl.org/Homo_sapiens/Transcript/SupportingEvidence?db=core;g=ENSG00000165264;r=9:32552997-32573160;t=ENST00000379847
> NM_001204090.1
Same problem as above, we did not use this sequence.
What you can do is to use the HGNC identifier of these failing IDs in the fetch_all_by_external_id method, i.e. NM_001199987.1 -> NDUFB6
Regards
Thibaut
> NM_001242881.1
> NM_014249.2
> NM_015584.3
> NM_024728.2
>
> Can you tell me how to retrieve these from the database?
>
> Here is the portion of my script I use to retrieve the data:
>
> foreach my $query_transcript (@transcripts_of_interest) {
> chomp $query_transcript;
> my $transcript = "";
> if ($query_transcript =~ /ENST/i){
> $transcript = $transcript_adaptor->fetch_by_stable_id("$query_transcript");
> }
> else{
> ($transcript) = @{ $transcript_adaptor->fetch_all_by_external_name("$query_transcript”);
> }
>
> unless ($transcript){
> $progress->message("Query: $query_transcript failed");
> next;
> }
> foreach my $exon ( @{ $transcript->get_all_Exons() } ) {
> my $estring = feature2string($exon);
> print "$query_transcript:\t$estring\n";
> }
> $next_update = $progress->update() if (++$j > $next_update);
>
> }
>
> Best regards
>
> Duarte Molha
>
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