[ensembl-dev] Compara: project from Human to Mouse
Benoît Ballester
benoit.ballester at inserm.fr
Tue Dec 4 14:04:45 GMT 2012
I may have found the solution myself.
I used align_slices to get coordinates of my human slice on the Genomic Align Block /Genomic Align.
Then I used those coordinates to restrict the human and mouse genomic_aligns
Ben
On 3 Dec 2012, at 19:00, Benoît Ballester wrote:
> Dear Compara,
>
> I have a few slices (~100bp) I'd like to project from human to mouse using the BLASTZ alignment (I am on v65).
> Looking at the example scripts, and digging into my memories, I got to the point where I have the genomic_align objects.
>
> However I'd like to have the Mouse coordinates (the exact projection from human, ~100bp) and not the entire genomic_align.
> I remember that there is another step to get the exact mouse coordinates.
>
> Would you please remind me what this next step is ?
>
> Thanks,
>
> #--8< ----Example script
>
> my $gabs = $gab_adaptor->fetch_all_by_MethodLinkSpeciesSet_Slice($hsap_mmus_blastz_mlss, $hsap_slice);
>
> foreach my $gab (@$gabs) {
>
> my $genomic_aligns = $gab->get_all_GenomicAligns();
>
> foreach my $genomic_align (@$genomic_aligns) {
> print
> $genomic_align->dnafrag->genome_db->name, " ",
> $genomic_align->dnafrag->coord_system_name, " ",
> $genomic_align->dnafrag->name, " [",
> $genomic_align->dnafrag_start, " - ",
> $genomic_align->dnafrag_end, "] ",
> $genomic_align->dnafrag_strand, #")\n",
> $genomic_align->dnafrag_end - $genomic_align->dnafrag_start, "\n",
> "\n"
> }
> print "\n";
> }
>
>
> #-- Example output
> Line: chr1 5482670 5482784 5482727
> homo_sapiens chromosome 1 [5482702 - 5484342] 11640
> mus_musculus chromosome 4 [152209531 - 152211053] -11522
>
>
>
> Ben
> wxw
>
>
> --
> Benoît Ballester, PhD
> Inserm U1090, TAGC
> Marseille - France
> +33 4 91 82 87 39
>
>
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--
Benoît Ballester, PhD
Inserm U1090, TAGC
Marseille - France
+33 4 91 82 87 39
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