[ensembl-dev] FW: ensembl api query

Monika Komorowska monika at ebi.ac.uk
Mon Jun 13 16:04:39 BST 2011


Hi David

Sorry about this, but it looks like we had the wrong version of DBEntryAdaptor checked in to branch 62. 
I have checked in the correct version, please checkout the DBEntryAdaptor module again.

Thanks
Monika

On 13 Jun 2011, at 14:44, BURT David wrote:

> Hi Monika
>  
> I still get the wrong version
> With same error about add_linkage_type
>  
> When you look at DBEntryAdaptor.pm what do you find at line 1262 and 1263?
>  
> I am sorry I can’t understand how CVS picks up the wrong version?
>  
> Dave
>  
> Professor David W. Burt
> Chair of Comparative Genomics
> Dept. Genomics and Genetics
> The Roslin Institute and Royal (Dick) School of Veterinary  Studies
> The Roslin Institute Building
> University of Edinburgh
> Easter Bush Campus
> Midlothian EH25 9RG
>  
> Phone: +44 (0)131 651 9216
> Lab:   +44 (0)131 651 9380
> Fax:   +44 (0)131 651 9105
> Email: Dave.Burt at roslin.ed.ac.uk
> WWW site: http://www.roslin.ed.ac.uk/dave-burt/
> The 6th International chick meeting - Edinburgh: http://www.roslin.ed.ac.uk/chick6/
>  
> Roslin Institute is a company limited by guarantee, registered in Scotland (registered number SC157100) and a Scottish Charity (registered number SC023592). Our registered office is at Roslin, Midlothian, EH25 9PS. VAT registration number 847380013.
>  
> The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only.   The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute
>  
> From: Monika Komorowska [mailto:monika at ebi.ac.uk] 
> Sent: 13 June 2011 14:18
> To: BURT David
> Cc: dev at ensembl.org
> Subject: Re: [ensembl-dev] FW: ensembl api query
>  
> Hi David
>  
> Would you mind creating a new sub-directory say ensembl_v62 in your home directory, cd into it and checkout version 62 again, using this command:
> $ cvs -d :pserver:cvsuser at cvs.sanger.ac.uk:/cvsroot/ensembl checkout -r branch-ensembl-62 ensembl-api
>  
> Add the new path to the PERL5LIB variable in your .cshrc file:
> setenv PERL5LIB ${PERL5LIB}:${HOME}/ensembl_v62/ensembl/modules
> and delete any other paths to ensembl/modules
>  
> You'll need to source the .cshrc file 
> Hopefully this time your script will pick up the correct version of the API
>  
> Thanks
> Monika
>  
>  
> On 13 Jun 2011, at 14:02, BURT David wrote:
> 
> 
> Hi Monika,
>  
> Many thanks – I think we are getting close.
>  
> 1_Attached is my copy of DBEntryAdaptor found in D:\Bioinformatics\Ensembl\src\ensembl\modules\Bio\EnsEMBL\DBSQL
> As you can see “add_linkage_type” is at line 1263 in DBEntryAdaptor in this version 62
>  
> Here’s an extract [since file too big for dev list]
>  
>       if (    defined($linkage_type)
>            && $linkage_type ne ""
>            && !$linkage_types{$refID}->{$linkage_key} )
>       {
>         $source_xref = ( defined($source_xref_id)
>                             ? $self->fetch_by_dbID($source_xref_id)
>                             : undef );
>         $seen{$refID}
>           ->add_linkage_type( $linkage_type, $source_xref || () );
>         $linkage_types{$refID}->{$linkage_key} = 1;
>       }
>     } ## end while ( my $arrRef = shift...
>   } ## end while ( my $rowcache = $sth...
>  
> 2_This was downloaded using instructions from Ensembl (see below) using CVS
>  
> $ cvs -d :pserver:cvsuser at cvs.sanger.ac.uk:/cvsroot/ensembl login
> Logging in to :pserver:cvsuser at cvs.sanger.ac.uk:2401/cvsroot/ensembl
> CVS password: CVSUSER
>  
> NB: You can install as many Ensembl APIs as you need in this way. To install all the APIs in one go, use the command:
> $ cvs -d :pserver:cvsuser at cvs.sanger.ac.uk:/cvsroot/ensembl checkout -r branch-ensembl-62 ensembl-api
>  
> 3_However, I found an old install – based on version 56 – this worked up to version 59, but software failed after that.
>  
> Any more thoughts? Looks like my version 62 is wrong?
>  
> Dave
>  
> Professor David W. Burt
> Chair of Comparative Genomics
> Dept. Genomics and Genetics
> The Roslin Institute and Royal (Dick) School of Veterinary  Studies
> The Roslin Institute Building
> University of Edinburgh
> Easter Bush Campus
> Midlothian EH25 9RG
>  
> Phone: +44 (0)131 651 9216
> Lab:   +44 (0)131 651 9380
> Fax:   +44 (0)131 651 9105
> Email: Dave.Burt at roslin.ed.ac.uk
> WWW site: http://www.roslin.ed.ac.uk/dave-burt/
> The 6th International chick meeting - Edinburgh: http://www.roslin.ed.ac.uk/chick6/
>  
> Roslin Institute is a company limited by guarantee, registered in Scotland (registered number SC157100) and a Scottish Charity (registered number SC023592). Our registered office is at Roslin, Midlothian, EH25 9PS. VAT registration number 847380013.
>  
> The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only.   The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute
>  
> From: Monika Komorowska [mailto:monika at ebi.ac.uk] 
> Sent: 13 June 2011 11:01
> To: BURT David
> Cc: dev at ensembl.org
> Subject: Re: [ensembl-dev] FW: ensembl api query
>  
> Hi David
>  
> Are you sure you're using version 62, I just tried your script on 62 and didn't get the error.
> I can't see a call to add_linkage_type at line 1262 in DBEntryAdaptor in version 62 either.
>  
> Can you add these two lines to your code, to get the API version you're running:
> use Bio::EnsEMBL::ApiVersion;
>   printf( "The API version used is %s\n", software_version() );
>  
>  
> Regards
> Monika
>  
> On 12 Jun 2011, at 18:46, BURT David wrote:
>  
> 
> any more ideas on problem below?
> 
> made a few changes - still not working on my system (tried two PCs)
> 
> interesting - I can get $dbe->display_id() but nothing from $dbe->dbname()
> also able to get versions, release and other parameters but no dbname (this is w=oine of the things I wanted)
> so looks like the external database name is not being assigned
> 
> however Bert and Daniel get the simple script to work
> 
> I have re-sinatlled v62 - still odd behaviour
> 
> "Can't locate object method "add_linkage_type" via package "Bio::EnsEMBL::IdentityXref"
> at /cygdrive/c/Bioinformatics/Ensembl/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm line 1262."
> 
> where there is no add_linkage_type metrhod in Bio::EnsEMBL::IdentityXref
> 
> so I think must be earlier problem related to dbname not being assigned
> 
> must be something with my setup?
> 
> 
> 
> Recently ran a "simple" test of my Ensembl perl api install using an example from the core tutorial - to list external database entries.
> 
> Perl program and output is attached and below.
> 
> PERL SCRIPT
> ==========
> 
> #!/bin/perl.exe -w
> 
> use strict;
> 
> use Bio::EnsEMBL::Registry;
> my $registry = 'Bio::EnsEMBL::Registry';
> $registry->load_registry_from_db(-host => 'ensembldb.ensembl.org', -user => 'anonymous');
> 
> print $gene_adaptor->dbc->dbname."\n";
> print $gene_adaptor->dbc->port."\n\n";
> 
> # Define a helper subroutine to print DBEntries
> sub print_DBEntries
> {
>    my $db_entries = shift;
> 
>    foreach my $dbe ( @{$db_entries} ) {
>        printf "\tXREF %s (%s)\n", $dbe->display_id(), $dbe->dbname();
>    }
> }
> 
> my $gene_adaptor = $registry->get_adaptor( 'Human', 'Core', 'Gene' );
> 
> # Get the 'COG6' gene from human
> my $gene = $gene_adaptor->fetch_by_display_label('COG6');
> 
> print "GENE ", $gene->stable_id(), "\n";
> print_DBEntries( $gene->get_all_DBEntries() );
> 
> foreach my $transcript ( @{ $gene->get_all_Transcripts() } ) {
>    print "TRANSCRIPT ", $transcript->stable_id(), "\n";
>    print_DBEntries( $transcript->get_all_DBEntries() );
> 
>    # Watch out: pseudogenes have no translation
>    if ( defined $transcript->translation() ) {
>        my $translation = $transcript->translation();
> 
>        print "TRANSLATION ", $translation->stable_id(), "\n";
>        print_DBEntries( $translation->get_all_DBEntries() );
>    }
> }
> 
> 
> OUTPUT
> ======
> 
> homo_sapiens_core_62_37g
> 5306
> 
> GENE ENSG00000133103
> Use of uninitialized value in printf at test.pl line 39.
>        XREF OTTHUMG00000016768 ()
> Use of uninitialized value in printf at test.pl line 39.
>        XREF COG6 ()
> TRANSCRIPT ENST00000416691
> 
> Can't locate object method "add_linkage_type" via package "Bio::EnsEMBL::IdentityXref"
> at /cygdrive/c/Bioinformatics/Ensembl/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm line 1262.
> 
> 
> 
> -- 
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
> 
> <dbentry_errors.txt><dbentry_test.pl>_______________________________________________
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>  
> Monika Komorowska
> EnsEMBL Software Developer
>  
> European Bioinformatics Institute (EMBL-EBI)
> tel: +44(0) 1233 494 409
>  
>  
> Monika Komorowska
> EnsEMBL Software Developer
>  
> European Bioinformatics Institute (EMBL-EBI)
> tel: +44(0) 1233 494 409
>  

Monika Komorowska
EnsEMBL Software Developer

European Bioinformatics Institute (EMBL-EBI)
tel: +44(0) 1233 494 409

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