Hi , Just wanted to what is provided by TranscriptVariationAllele::polyphen_score Is this the pph2_prob 'classifier probability of the variation being damaging', as described here ?: http://genetics.bwh.harvard.edu/pph2/dokuwiki/appendix_a Many Thanks -- Chris Joyce Team 136, Medical Sequencing Wellcome Trust Sanger Institute Tel: 01223 834244 Ext. 6859