[ensembl-dev] Few queries

[Anas Al Okaily]

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Dear VEP developers,

       This is Anas Al-okaily a Bioinformatist from King Hussein Cancer Center, Amman, Jordan. We have 137 vcf file called from whole-genome sequencing data (GRCH38 assembly), and we need to annotate them using VEP tool against several databases (most of them are provided as plugins by VEP).


I tried to install some of these plugins but I faced issues in finding the files that need to be download and faced that some of these plugins need installation (which I could not find out how-to). Alternatively, I looked into VEP tool and found it contains all annotations that we need. However, when I tried to upload the vcf file one-by-one (where the size of each file on average is about 3GB), it was not inputted (hence annotated) and it raised "Unknow error".


Is there is a size limitation on the VCF file that can be accepted by VEP webtool? If so, can you please help me in tackling this important matter for us please by maybe guiding me how to install the needed plugins or by any other solution?


Best regards



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