[ensembl-dev] Fwd: Ensembl VEP: FASTA gzip to bgzip conversion failed
Platon workaccount
platon.work at gmail.com
Sun Apr 10 20:38:43 BST 2016
Hello!
~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio$ ls
DB HTS.c HTS.o HTS.pm HTS.xs
~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/DB$ ls
HTS
~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/DB/HTS$ ls
Alignment.pm AlignWrapper.pm Constants.pm Faidx.c Faidx.o Faidx.pm Faidx.xs
FetchIterator.pm Pileup.pm PileupWrapper.pm Query.pm ReadIterator.pm
Segment.pm Tabix Tabix.pm Target.pm VCF.pm
вт, 5 апр. 2016 г. в 11:01, Rishi Nag <rishi at ebi.ac.uk>:
>
> Can I have listings for the following directories please?
> ~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/
> ~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/DB
> ~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/DB/HTS
>
> Regards
> Rishi
>
> On 31/03/2016 16:50, Platon workaccount wrote:
> > After that, command 'perl biodbhts/t/00_load.t' yields the same result.
> >
> > чт, 31 мар. 2016 г. в 16:30, Rishi Nag <rishi at ebi.ac.uk <mailto:
> rishi at ebi.ac.uk>>:
> >
> >
> > Hi
> >
> > Could you try moving
> > ~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/HTS
> > into
> >
> ~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/DB/HTS
> > and give it another go please?
> >
> > Regards
> >
> > Rishi
> >
> > On 31/03/16 11:33, Platon workaccount wrote:
> > >
> :~/ensembl-tools-release-84/scripts/variant_effect_predictor/biodbhts$ ls
> > > blib _build Build Build.PL Changes cpanfile DISCLAIMER INSTALL.pl
> lib
> > > LICENSE MANIFEST MANIFEST.SKIP META.json META.yml MYMETA.json
> MYMETA.yml
> > > ppport.h README scripts t typemap
> > >
> > >
> :~/ensembl-tools-release-84/scripts/variant_effect_predictor/Bio/HTS$ ls
> > > Alignment.pm AlignWrapper.pm Constants.pm Faidx.c Faidx.o Faidx.pm
> > > Faidx.xs FetchIterator.pm Pileup.pm PileupWrapper.pm Query.pm
> > > ReadIterator.pm Segment.pm Tabix Tabix.pm Target.pm VCF.pm
> > >
> > > perl biodbhts/t/00_load.t
> > >
> > >
> > > 1..3
> > >
> > > not ok 1 - use Bio::DB::HTS;
> > >
> > > # Failed test 'use Bio::DB::HTS;'
> > >
> > > # at biodbhts/t/00_load.t line 3.
> > >
> > > # Tried to use 'Bio::DB::HTS'.
> > >
> > > # Error: Can't locate Bio/DB/HTS.pm in @INC (you may need to
> install the
> > > Bio::DB::HTS module) (@INC contains: /home/pl/src/bioperl-live
> > > /home/pl/src/ensembl/modules /home/pl/src/ensembl-compara/modules
> > > /home/pl/src/ensembl-variation/modules
> > > /home/pl/src/ensembl-funcgen/modules
> > > /home/pl/src//lib/x86_64-linux-gnu/perl/5.22.1/
> > > /home/pl/src/ensembl-io/modules
> > > /home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.22.1
> > > /usr/local/share/perl/5.22.1 /usr/lib/x86_64-linux-gnu/perl5/5.22
> > > /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.22
> > > /usr/share/perl/5.22 /usr/local/lib/site_perl
> > > /usr/lib/x86_64-linux-gnu/perl-base .) at biodbhts/t/00_load.t
> line 3.
> > >
> > > # BEGIN failed--compilation aborted at biodbhts/t/00_load.t line
> 3.
> > >
> > > not ok 2 - use Bio::DB::HTS::Tabix;
> > >
> > > # Failed test 'use Bio::DB::HTS::Tabix;'
> > >
> > > # at biodbhts/t/00_load.t line 4.
> > >
> > > # Tried to use 'Bio::DB::HTS::Tabix'.
> > >
> > > # Error: Can't locate Bio/DB/HTS/Tabix.pm in @INC (you may need to
> > > install the Bio::DB::HTS::Tabix module) (@INC contains:
> > > /home/pl/src/bioperl-live /home/pl/src/ensembl/modules
> > > /home/pl/src/ensembl-compara/modules
> > > /home/pl/src/ensembl-variation/modules
> > > /home/pl/src/ensembl-funcgen/modules
> > > /home/pl/src//lib/x86_64-linux-gnu/perl/5.22.1/
> > > /home/pl/src/ensembl-io/modules
> > > /home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.22.1
> > > /usr/local/share/perl/5.22.1 /usr/lib/x86_64-linux-gnu/perl5/5.22
> > > /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.22
> > > /usr/share/perl/5.22 /usr/local/lib/site_perl
> > > /usr/lib/x86_64-linux-gnu/perl-base .) at biodbhts/t/00_load.t
> line 4.
> > >
> > > # BEGIN failed--compilation aborted at biodbhts/t/00_load.t line
> 4.
> > >
> > > not ok 3 - use Bio::DB::HTS::Tabix::Iterator;
> > >
> > > # Failed test 'use Bio::DB::HTS::Tabix::Iterator;'
> > >
> > > # at biodbhts/t/00_load.t line 5.
> > >
> > > # Tried to use 'Bio::DB::HTS::Tabix::Iterator'.
> > >
> > > # Error: Can't locate Bio/DB/HTS/Tabix/Iterator.pm in @INC (you
> may need
> > > to install the Bio::DB::HTS::Tabix::Iterator module) (@INC
> contains:
> > > /home/pl/src/bioperl-live /home/pl/src/ensembl/modules
> > > /home/pl/src/ensembl-compara/modules
> > > /home/pl/src/ensembl-variation/modules
> > > /home/pl/src/ensembl-funcgen/modules
> > > /home/pl/src//lib/x86_64-linux-gnu/perl/5.22.1/
> > > /home/pl/src/ensembl-io/modules
> > > /home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.22.1
> > > /usr/local/share/perl/5.22.1 /usr/lib/x86_64-linux-gnu/perl5/5.22
> > > /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.22
> > > /usr/share/perl/5.22 /usr/local/lib/site_perl
> > > /usr/lib/x86_64-linux-gnu/perl-base .) at biodbhts/t/00_load.t
> line 5.
> > >
> > > # BEGIN failed--compilation aborted at biodbhts/t/00_load.t line
> 5.
> > >
> > > # Looks like you failed 3 tests of 3.
> > >
> > >
> > >
> > > чт, 31 мар. 2016 г. в 13:15, Rishi Nag <rishi at ebi.ac.uk <mailto:
> rishi at ebi.ac.uk>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>:
> > >
> > >
> > > The failure occurs as the script attempts to 'use
> Bio::DB::HTS::Faidx'
> > > to perform the conversion but then can't load it.
> > >
> > > So does
> > >
> /home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor have
> > > a subdirectory Bio/DB/HTS and if so can you provide an ls of
> this
> > > directory please?
> > >
> > > And then from
> > >
> /home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > try running
> > > perl biodbhts/t/00_load.t
> > >
> > > This should indicate a more in-depth nature of what is
> causing the
> > > error,
> > >
> > > Regards
> > >
> > > Rishi
> > >
> > >
> > >
> > > On 31/03/16 10:18, Platon workaccount wrote:
> > > >
> > >
> PERL5LIB=${PERL5LIB}:${HOME}/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > > export PERL5LIB
> > > > perl INSTALL.pl
> > > >
> > > > <...>
> > > > Indexing failed - VEP will attempt to index the file the
> first
> > > time you
> > > > use it
> > > > <...>
> > > >
> > > > echo $PERL5LIB
> > > >
> > > >
> > >
> >
> :/home/pl/src/bioperl-live:/home/pl/src/ensembl/modules:/home/pl/src/ensembl-compara/modules:/home/pl/src/ensembl-variation/modules:/home/pl/src/ensembl-funcgen/modules:/home/pl/src//lib/x86_64-linux-gnu/perl/5.22.1/:/home/pl/src/ensembl-io/modules:/home/pl/ensembl-tools-release-84/scripts/variant_effect_predictor
> > > >
> > > > чт, 31 мар. 2016 г. в 11:24, Rishi Nag <rishi at ebi.ac.uk
> <mailto:rishi at ebi.ac.uk>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>:
> > > >
> > > > Hi There
> > > >
> > > > Thanks for the information. The request should have
> been 'echo
> > > > $PERL5LIB', but it looks like (from the commands below
> to set the
> > > > PERL5LIB environment variable) that things have been
> set to
> > > install
> > > > the API in the ${HOME}/src directory. If you also add
> the
> > > directory
> > > > containing the Bio directory and the so files that
> were copied to
> > > > the PERL5LIB variable it should get picked up,
> > > >
> > > > Rishi
> > > >
> > > > On 30/03/16 19:09, Platon workaccount wrote:
> > > > > Hello!
> > > > >
> > > > > I tried the second set.
> > > > >
> > > > > echo PERL5LIB
> > > > > PERL5LIB
> > > > >
> > > > > Full command history:
> > > > >
> > > > > sudo cpan DBI
> > > > > sudo apt-get install libmysqlclient-dev
> > > > > sudo cpan DBD::mysql
> > > > > sudo apt-get install git
> > > > > cd src
> > > > > git clonehttps://
> github.com/bioperl/bioperl-live.git <
> http://github.com/bioperl/bioperl-live.git>
> > > <http://github.com/bioperl/bioperl-live.git>
> > > > <http://github.com/bioperl/bioperl-live.git>
> > > > > cd bioperl-live
> > > > > git checkout release-1-6-924
> > > > > cd -
> > > > > git clonehttps://
> github.com/Ensembl/ensembl-git-tools.git <
> http://github.com/Ensembl/ensembl-git-tools.git>
> > > <http://github.com/Ensembl/ensembl-git-tools.git>
> > > > <http://github.com/Ensembl/ensembl-git-tools.git>
> > > > > export PATH=$PWD/ensembl-git-tools/bin:$PATH
> > > > > git ensembl --clone api
> > > > > PERL5LIB=${PERL5LIB}:${HOME}/src/bioperl-live
> > > > > PERL5LIB=${PERL5LIB}:${HOME}/src/ensembl/modules
> > > > >
> PERL5LIB=${PERL5LIB}:${HOME}/src/ensembl-compara/modules
> > > > >
> PERL5LIB=${PERL5LIB}:${HOME}/src/ensembl-variation/modules
> > > > >
> PERL5LIB=${PERL5LIB}:${HOME}/src/ensembl-funcgen/modules
> > > > > export PERL5LIB
> > > > > git clonehttps://github.com/samtools/tabix.git <
> http://github.com/samtools/tabix.git>
> > > <http://github.com/samtools/tabix.git>
> > > > <http://github.com/samtools/tabix.git>
> > > > > cd tabix
> > > > > make
> > > > > cd perl
> > > > > perl Makefile.PL PREFIX=${HOME}/src/
> > > > > make && make install
> > > > >
> > >
> PERL5LIB=${PERL5LIB}:${HOME}/src//lib/x86_64-linux-gnu/perl/5.22.1/
> > > > > export PERL5LIB
> > > > > PATH=${PATH}:${HOME}/src/tabix/
> > > > > export PATH
> > > > > PERL5LIB=${PERL5LIB}:${HOME}/src/ensembl-io/modules
> > > > > export PERL5LIB
> > > > > cd ~
> > > > > cd
> ensembl-tools-release-84/scripts/variant_effect_predictor/
> > > > > git clone -b master
> > > --depth=1https://github.com/samtools/htslib.git
> > > > > cd htslib
> > > > > make
> > > > > export HTSLIB_DIR="$PWD"
> > > > > cd ..
> > > > > git clonehttps://github.com/Ensembl/Bio-HTS.git <
> http://github.com/Ensembl/Bio-HTS.git>
> > > <http://github.com/Ensembl/Bio-HTS.git>
> > > > <http://github.com/Ensembl/Bio-HTS.git> biodbhts
> > > > > cd biodbhts/
> > > > > sudo cpan Module::Build
> > > > > perl Build.PL
> > > > > ./Build
> > > > > cp blib/arch/auto/Bio/DB/HTS/Faidx/Faidx.so ..
> > > > > cp blib/arch/auto/Bio/DB/HTS/HTS.so ..
> > > > > cp -r lib/Bio/* ../Bio/
> > > > > cd ~
> > > > > cd
> ensembl-tools-release-84/scripts/variant_effect_predictor/
> > > > > sudo cpan Archive::Extract
> > > > > perl INSTALL.pl
> > > > >
> > > > >
> > > > > вт, 29 мар. 2016 г. в 13:35, Rishi Nag <
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>:
> > > > >
> > > > >
> > > > > Hi
> > > > >
> > > > > Sorry to hear that and sorry for not replying
> earlier
> > > due to the
> > > > > Easter break.
> > > > >
> > > > > Did you try the sudo set of instructions or the
> second
> > > set?
> > > > And can
> > > > > I check what your PERL5LIB is set to please
> (run 'echo
> > > > PERL5LIB')?
> > > > >
> > > > > Regards
> > > > >
> > > > > Rishi
> > > > >
> > > > > On 23/03/2016 19:42, Platon workaccount wrote:
> > > > > > Unfortunately, your instructions did not
> help.
> > > > > >
> > > > > > Installation log:
> > > > > >
> > > > > >
> > > > > > $ perl INSTALL.pl
> > > > > >
> > > > > >
> > > > > > Hello! This installer is configured to
> install v84
> > > of the
> > > > Ensembl
> > > > > API for use by the VEP.
> > > > > >
> > > > > > It will not affect any existing
> installations of the
> > > > Ensembl API
> > > > > that you may have.
> > > > > >
> > > > > >
> > > > > > It will also download and install cache
> files from
> > > > Ensembl's FTP
> > > > > server.
> > > > > >
> > > > > >
> > > > > > Checking for installed versions of the
> Ensembl
> > > > API...Subroutine
> > > > > Tabix::tabix_open redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > > > > line 17.
> > > > > >
> > > > > > Subroutine Tabix::tabix_close redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > line 17.
> > > > > >
> > > > > > Subroutine Tabix::tabix_query redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > line 17.
> > > > > >
> > > > > > Subroutine Tabix::tabix_read redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > line 17.
> > > > > >
> > > > > > Subroutine Tabix::tabix_getnames redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > line 17.
> > > > > >
> > > > > > Subroutine TabixIterator::tabix_iter_free
> redefined at
> > > > >
> /home/pl/src/lib/x86_64-linux-gnu/perl/5.20.2/Tabix.pm
> > > line 17.
> > > > > >
> > > > > > done
> > > > > >
> > > > > > It looks like you already have v84 of the API
> > > installed.
> > > > > >
> > > > > > You shouldn't need to install the API
> > > > > >
> > > > > >
> > > > > > Skip to the next step (n) to install cache
> files
> > > > > >
> > > > > >
> > > > > > Do you want to continue installing the API
> (y/n)? n
> > > > > >
> > > > > > - skipping API installation
> > > > > >
> > > > > >
> > > > > > The VEP can either connect to remote or local
> > > databases,
> > > > or use
> > > > > local cache files.
> > > > > >
> > > > > > Using local cache files is the fastest and
> most
> > > efficient
> > > > way to
> > > > > run the VEP
> > > > > >
> > > > > > Cache files will be stored in /home/pl/.vep
> > > > > >
> > > > > > Do you want to install any cache files
> (y/n)? n
> > > > > >
> > > > > > Skipping cache installation
> > > > > >
> > > > > >
> > > > > > The VEP can use FASTA files to retrieve
> sequence
> > > data for HGVS
> > > > > notations and reference sequence checks.
> > > > > >
> > > > > > FASTA files will be stored in /home/pl/.vep
> > > > > >
> > > > > > Do you want to install any FASTA files
> (y/n)? y
> > > > > >
> > > > > > FASTA files for the following species are
> > > available; which
> > > > do you
> > > > > want (can specify multiple separated by spaces,
> "0" to
> > > > install for
> > > > > species specified
> > > > > > for cache download):
> > > > > >
> > > > > > <...>
> > > > > >
> > > > > > 28 : homo_sapiens
> > > > > >
> > > > > > <...>
> > > > > >
> > > > > > ? 28
> > > > > >
> > > > > > - downloading
> > > Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
> > > > > >
> > > > > > - converting sequence data to bgzip format
> > > > > >
> > > > > > Going to run:
> > > > > >
> > > > > > ./biodbhts/scripts/convert_gz_2_bgz.sh
> > > > >
> > > >
> > >
> /home/pl/.vep/homo_sapiens/84_GRCh38/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
> > > > > ./htslib/bgzip
> > > > > >
> > > > > > This may take some time and will be removed
> when
> > > files are
> > > > > provided in bgzip format
> > > > > >
> > > > > > Converted FASTA gzip file to bgzip
> successfully
> > > > > >
> > > > > > Indexing failed - VEP will attempt to index
> the
> > > file the first
> > > > > time you use it
> > > > > >
> > > > > > The FASTA file should be automatically
> detected by
> > > the VEP
> > > > when
> > > > > using --cache or --offline. If it is not, use
> "--fasta
> > > > > >
> > > > >
> > > >
> > >
> /home/pl/.vep/homo_sapiens/84_GRCh38/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz"
> > > > > >
> > > > > >
> > > > > > The VEP can use plugins to add functionality
> and data.
> > > > > >
> > > > > > Plugins will be installed in
> /home/pl/.vep/Plugins
> > > > > >
> > > > > > Do you want to install any plugins (y/n)? n#
> #y
> > > > > >
> > > > > > ** GET
> > > > >
> > > >
> > >
> https://raw.githubusercontent.com/Ensembl/VEP_plugins/release/84/plugin_config.txt
> > > > > ==> 16%#### 32%#### 48%#### 64%#### 80%####
> > > > > > 96%####100%####200 OK
> > > > > >
> > > > > >
> > > > > > ср, 23 мар. 2016 г. в 19:49, Rishi Nag
> > > <rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>>:
> > > > > >
> > > > > >
> > > > > > It sounds like there has been a failure
> to set
> > > up . If you
> > > > > could pass on the full output from your install
> attempt
> > > > relating to
> > > > > the API components that
> > > > > > would be useful.
> > > > > >
> > > > > > There are two ways forward however to
> complete the
> > > > > installation process manually.
> > > > > >
> > > > > > The first is if you have sudo
> permissions on your
> > > > machine and
> > > > > are happy to have libraries installed on the
> system level:
> > > > > > cd htslib
> > > > > > sudo make install
> > > > > > cd ../biodbhts
> > > > > > perl Build.PL
> > > > > > ./Build
> > > > > > sudo ./Build install
> > > > > > cd ..
> > > > > >
> > > > > >
> > > > > > Alternatively the following replicates
> what the
> > > INSTALL
> > > > > script should be doing:
> > > > > > cd htslib
> > > > > > export HTSLIB_DIR="$PWD"
> > > > > > cd ../biodbhts
> > > > > > perl Build.PL
> > > > > > ./Build
> > > > > > cp
> blib/arch/auto/Bio/DB/HTS/Faidx/Faidx.so ..
> > > > > > cp blib/arch/auto/Bio/DB/HTS/HTS.so ..
> > > > > > cp -r lib/Bio/* ../Bio/
> > > > > >
> > > > > > And finally, if these cause issues there
> is the
> > > option of
> > > > > re-installing with the -NO_HTSLIB flag, which
> will
> > > bypass the
> > > > > bgzipped sequence file
> > > > > > process.
> > > > > >
> > > > > > Regards
> > > > > >
> > > > > > Rishi
> > > > > >
> > > > > >
> > > > > > On 23/03/2016 14:52, Platon workaccount
> wrote:
> > > > > > > Thanks! "Converted FASTA gzip file to
> bgzip
> > > > successfully",
> > > > > but this time "Indexing failed - VEP will
> attempt to index
> > > > the file
> > > > > the first time
> > > > > > you use
> > > > > > > it". Is it a critical error?
> > > > > > >
> > > > > > > ср, 23 мар. 2016 г. в 12:02, Rishi Nag
> > > > <rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>>>:
> > > > > > >
> > > > > > >
> > > > > > > Thanks.
> > > > > > >
> > > > > > > Change into the same directory as
> INSTALL.pl
> > > > > > >
> > > > > > > First check for a htslib
> directory being
> > > > present with
> > > > > the ls command. If not then download and
> compile this:
> > > > > > > git clone -b master --depth=1
> > > > > https://github.com/samtools/htslib.git
> > > > > > > cd htslib
> > > > > > > make
> > > > > > > cd ..
> > > > > > >
> > > > > > > git clone
> > > > https://github.com/Ensembl/Bio-HTS.git biodbhts
> > > > > > > You should then be able to re-run
> the
> > > install
> > > > commands
> > > > > to download the sequence from this directory.
> > > > > > >
> > > > > > > Regards
> > > > > > >
> > > > > > > Rishi
> > > > > > >
> > > > > > >
> > > > > > > On 22/03/2016 18:17, Platon
> workaccount
> > > wrote:
> > > > > > > > VEP's install script was
> invoked by
> > > command perl
> > > > > INSTALL.pl
> > > > > > > >
> > > > > > > > вт, 22 мар. 2016 г. в 15:32,
> Rishi Nag
> > > > > <rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>>
> > > > > > <mailto:rishi at ebi.ac.uk <mailto:
> rishi at ebi.ac.uk>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>
> > > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>
> > > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>
> <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>
> > > <mailto:rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk> <mailto:
> rishi at ebi.ac.uk <mailto:rishi at ebi.ac.uk>>>>>>>>:
> > > > > > > >
> > > > > > > >
> > > > > > > > Hi
> > > > > > > >
> > > > > > > > Thanks for raising this
> issue.
> > > Can you
> > > > provide
> > > > > details on how the install script was invoked
> please?
> > > > > > > >
> > > > > > > > Regards
> > > > > > > >
> > > > > > > > Rishi
> > > > > > > >
> > > > > > > > On 22/03/2016 12:04, Platon
> > > workaccount
> > > > wrote:
> > > > > > > > >
> > > > > > > > >
> > > > > > > > > ---------- Forwarded
> message
> > > ---------
> > > > > > > > > From: Platon workaccount
> > > > > <platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>
> > > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>>>
> > > > > > > <mailto:platon.work at gmail.com
> <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>>>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>>>
> > > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>>>
> > > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com
> >>>
> > > > <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com> <mailto:platon.work at gmail.com <mailto:
> platon.work at gmail.com>>
> > > <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>
> <mailto:platon.work at gmail.com <mailto:platon.work at gmail.com>>>>>>
> > > >
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