[ensembl-dev] using VEP71 with mus_musculus release 67?
Paul Denny
paul.denny3 at gmail.com
Wed Jun 5 14:30:49 BST 2013
Dear VEP developers,
I have a pair of whole genome VCF format files in which variants were called against mus_musculus release 67 (assembly NCBI37 / mm9). I have no opportunity to take the raw data and re-align to mouse 71. I'd like to use VEP to look for the effects of the variants on genes and regulatory elements - can I use the latest version of VEP (71) or do I have to go back to versions 2.7 or 2.8?
I've tried VEP 71, using this command:
perl variant_effect_predictor.pl -i /Volumes/MacExternal/P110202/WTCHG_26483.bam.vcf -o VEP_BALB.txt -fork 4 -offline --regulatory --species mus_musculus --cache --dir /Users/p.denny/.vep/mus_musculus/67 --chr 1
but get error messages like this:
ERROR: Cache directory /Users/p.denny/.vep/mus_musculus/67/mus_musculus/71 not found at variant_effect_predictor.pl line 993.
many thanks,
Paul
Mobile: +44-07922078038
E-Mail: paul.denny3 at gmail.com
Web: http://www.gene-effects.co.uk
Twitter: http://twitter.com/pauldennyuk
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